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San José, Costa Rica, Thursday, May 5, 2016, Vol. 17, No. 88
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Independence expat celebration to be on a Sunday
By the A.M. Costa Rica staff

The U.S. Independence Day celebration will be July 3 at the Cervecería picnic grounds west of San José.

The sponsor, the American Colony Committee, said that the beer company, Florida Ice & Farm Co. has permitted the use of the picnic grounds even though the day was Sunday. In the past, the committee had to move the date to avoid a conflict with beer company employees on their day off.

Attendance is expected to be larger that Sunday than if the celebration was put off until the f
ollowing day, July 4. The committee
estimated about 3,000 attendees.

The committee is seeking donations again this year in anticipation of a larger turnout. Most U.S. expat firms and those that do business with expats generally make donations that are used to cover the expenses. Recently there has been a modest admission fee, but the committee continues to provide free beer and hot dogs in the style of a traditional U.S. Independence Day bash.

This is the 56th year for the picnic. The event was started to provide the children of expats a traditional July 4 celebration even though they were living here. More information is available at

Bacterium found to halt bulk of zika transmission
By the Cell Press news staff

Aedes mosquitoes carrying the bacterium Wolbachia, found inside the cells of 60 percent of all insect species, are drastically less able to transmit zika virus, say researchers at Brazil's Oswaldo Cruz Foundation in a study published Wednesday in Cell Host & Microbe.

This is the first report on the effect of Wolbachia bacteria on zika virus. Originally inserted into Aedes eggs as part of an eliminate dengue program, the bacterium is passed on from mother mosquitoes to offspring, so it is a sustainable control agent. The approach is already being piloted to control dengue virus transmission and, with the proper resources and approvals, there's infrastructure in place to increase the scale of current trials to also help tackle the zika epidemic.

Wolbachia bacteria were first identified in 2005 as a way to combat mosquito-borne infections. After four years, researchers were successful in their attempts to isolate the bacterium from fruit flies and get it inside Aedes mosquitoes' eggs, without using any genetic alteration. They expected Wolbachia to shorten mosquitoes' lifespans, but the bacterium provided an added bonus, in that it heavily reduced the dengue virus replication in the mosquito. The bacterium, it seems, has the same effect on zika transmission. The same effect was previously seen on chikungunya virus, also transmitted by Aedes mosquitoes.

"The idea has been to release Aedes mosquitoes with Wolbachia in the field over a period of a few months, so they mate with Aedes mosquitoes without Wolbachia living in the place and, over time, replace the mosquito population," says senior author Luciano
Moreira of the Oswaldo Cruz Foundation. He is also actively involved in the Eliminate Dengue Program, a non-profit that is testing the approach in 40 locations around the world.

"Zika and dengue belong in the same family of viruses, so with the outbreak in Brazil, the
logical idea was to test the mosquitoes carrying Wolbachia by challenging them with zika virus and see what would happen" he said.

Moreira's team gave Brazilian field mosquitoes and Wolbachia-infected 
Cell Press/Peter Iliccieve Fiocruz 
A researcher looking at Aedes mosquitoes infected with Wolbachia.

mosquitoes zika virus by feeding them human blood infected by two recent strains of the virus that is circulating in Brazil. After two weeks, the researchers saw that mosquitoes carrying Wolbachia had fewer viral particles in their bodies and saliva.

The tests showed that the virus present in the mosquito saliva was not active, meaning that, after biting, the mosquito would not be able to transmit zika virus. The reason for this drop in viral reproduction is unknown, but one theory is that because Wolbachia lives inside of the mosquito's cells, if the virus goes inside the cell to replicate, then there is an internal competition for resources. Surprisingly, this drop held true no matter how many Wolbachia the mosquito carried.

"Wolbachia showed to be as effective on zika as the most important dengue experiments we did," Moreira says. He cautions that the strategy is not 100 percent effective nor will it eliminate the virus. "We know that there will not be only one solution for zika. We have to do this alongside different approaches, like vaccines or insecticides, besides the public measures to control Aedes breeding sites."

He is currently discussing the Wolbachia approach with the Brazilian ministry of health, hoping to raise the resources and public support to test its effect on zika in the field.

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